Label-Free Detecting of the Compaction and Decompaction of ctDNA Molecules Induced by Surfactants with SERS Based on a nanoPAA-ZnCl<sub>2</sub>-AuLs Solid Substrate
Bojuan Hao, Kaige Wang, Yukun Zhou, Chaofan Sui, Lei Wang, Ren Bai, Zhaojin Yang
Abstract
M, and SERS spectra of ctDNA-CTAB and ctDNA-CTAB-SDS composites were collected, respectively. The interactions between ctDNA and surfactants were analyzed by changes in SERS spectra, for example, disappearances and appearances of SERS bands and relative changes of peak intensity, in which CTAB resulted in the compaction of the DNA molecule while SDS induced the decompaction of the ctDNA-CTAB complex. Moreover, UV-visible spectrophotometry was employed to demonstrate the compaction/decompaction of ctDNA molecules caused by surfactants. The local binding modes of ctDNA molecules and surfactant molecules were expounded. This work will be helpful for understanding biological processes such as DNA compaction and recombination within nucleus or/and cells and for the development of gene therapy technologies.