Coupling a rebuild shuttle system with biosynthetic pathway and transcription factor engineering for enhanced <scp>l</scp>-cysteine production
Bo Zhang, Xiao-Ling Xiu, Lifeng Chen, Xin Sui, Ruyi Zheng, Yuhe Guo, Xue Cai, Zhi‐Qiang Liu, Yu‐Guo Zheng
Abstract
A novel plasmid-free engineered E. coli strain was developed to significantly increase l -cysteine yield, achieving a titer of 35.54 g L −1 in a 5 L bioreactor.
Topics & Concepts
CysteineTranscription factorCoupling (piping)ChemistryBiosynthesisTranscription (linguistics)BiochemistryCell biologyBiologyEngineeringGeneEnzymeMechanical engineeringPhilosophyLinguisticsMetalloenzymes and iron-sulfur proteinsEndoplasmic Reticulum Stress and DiseaseRNA modifications and cancer