A Versatile Human Intestinal Organoid-Derived Epithelial Monolayer Model for the Study of Enteric Pathogens
Kourtney P. Nickerson, Alejandro Llanos‐Chea, Laura Ingano, Gloria Serena, Alba Miranda-Ribera, Meryl Perlman, Rosiane Lima, Marcelo B. Sztein, Alessio Fasano, Stefania Senger, Christina S. Faherty
Abstract
While traditional laboratory techniques and animal models have provided valuable knowledge in discerning virulence mechanisms of enteric pathogens, the complexity of the human gastrointestinal tract has hindered our understanding of physiologically relevant, human-specific interactions and, thus, has significantly delayed successful vaccine development. The human intestinal organoid-derived epithelial monolayer (HIODEM) model closely recapitulates the diverse cell populations of the intestine, allowing for the study of human-specific infections.
Topics & Concepts
OrganoidBiologyIntestinal epitheliumIntestinal mucosaEpitheliumComputational biologyMicrobiologyMedicineCell biologyInternal medicineGeneticsBacteriophages and microbial interactionsViral gastroenteritis research and epidemiologyCancer Cells and Metastasis