Collagen derived species-specific peptides for distinguishing donkey-hide gelatin (Asini Corii Colla)
Shuo Cai, Kexuan Zhao, Mengtong Jiang, Shuying Han, Yunfeng Zheng, Xun Liu, Ming Zhao, Jin‐Ao Duan, Rui Liu
Abstract
Objective: , ACC) has been used for thousands of years. However, till now few effective strategy had been proposed to distinguish ACC from other animal hide gelatins, especially closely related horse- and mule-hide gelatins, which was an embarrassment of ACC quality control. Methods: Combined mass spectrometry and bioinformatic methods have been applied to identify and verify two ACC-specific peptides (Pep-1 and Pep-2) capable of distinguishing ACC from other closely related animal gelatins with high selectivity. Results: It confirmed that these two peptides could be not only used for distinguishing ACC from highly homologous horse-hide and mule-hide gelatins as well as other animal hide gelatins. Conclusion: The present study provides a simple method for species-specific peptides discovery, which can be used for assessing the quality of animal gelatin products, and ensure they are authenticable and traceable.