Recent progresses in plant single-cell transcriptomics
Dihuai Zheng, Jiwei Xu, Yaqian Lu, Hongyu Chen, Qinjie Chu, Longjiang Fan
Abstract
High-throughput sequencing technologies at single-cell resolution have great potential to reveal a new landscape of plant cells. Single-cell/nucleus RNA (scRNA/snRNA), single-cell/nucleus assay for transposase accessible chromatin (scATAC/snATAC) and spatial transcriptome sequencing have been applied in multiple plant tissues. Consequently, a significant increase in publications on plant single-cell transcriptomics was seen in the recent two years. In this review, we will summarize the advantages and weaknesses of these single-cell sequencing approaches, offer a glimpse of their developments on cell biology, bioinformatic tools and databases in the latest two years.
Topics & Concepts
TranscriptomeComputational biologyBiologyChromatinSingle cell sequencingCellSingle-cell analysisTransposaseRNAGeneticsGenomeGeneGene expressionTransposable elementPhenotypeExome sequencingSingle-cell and spatial transcriptomicsGenomics and Phylogenetic StudiesCRISPR and Genetic Engineering