Ribosome-mediated incorporation of fluorescent amino acids into peptides <i>in vitro</i>
Joongoo Lee, Kevin J. Schwarz, Hao Yu, Antje Krüger, Eric V. Anslyn, Andrew D. Ellington, Jeffrey S. Moore, Michael C. Jewett
Abstract
We report the design, chemical synthesis, and flexizyme-catalyzed transfer RNA (tRNA) acylation of a variety of fluorescent amino acids (FAAs). The fluorescent groups include pyrene, coumarin, nitrobenzoxadiazole, and fluorescein variants. We further demonstrate site-specific incorporation of the FAAs into peptides by the ribosome in vitro through genetic code reprogramming.
Topics & Concepts
RibosomeFluorescenceAmino acidIn vitroChemistrySubstrate (aquarium)Scope (computer science)Fluorescent proteinBiochemistryTranslation (biology)Combinatorial chemistryGreen fluorescent proteinBiologyMessenger RNARNAComputer scienceGeneEcologyQuantum mechanicsPhysicsProgramming languageRNA and protein synthesis mechanismsChemical Synthesis and AnalysisMonoclonal and Polyclonal Antibodies Research