Calculating Apparent p <i>K</i> <sub>a</sub> Values of Ionizable Lipids in Lipid Nanoparticles
Nicholas B. Hamilton, Steve Arns, Mee Shelley, Irene Bechis, John C. Shelley
Abstract
High Resolution Image Download MS PowerPoint Slide Creating new ionizable lipids for use in lipid nanoparticles (LNPs) is an active field of research. One of the critical properties for selecting suitable ionizable lipids is the apparent p K a value of the lipid as formulated in an LNP. We have developed a structure-based, computational methodology for the prediction of the apparent p K a value of ionizable lipids within LNPs and have tested it using the lipid formulations in the mRNA LNP COVID-19 vaccines COMIRNATY and Spikevax, and the siRNA LNP therapeutic Onpattro. The calculation was also applied to Lipid A, a variant of the ionizable lipid used in COMIRNATY.
Topics & Concepts
ChemistryNanoparticleNanotechnologyMaterials scienceFree Radicals and AntioxidantsComputational Drug Discovery MethodsLipid Membrane Structure and Behavior