Multiple Single-Nucleotide Polymorphism Detection for Antimalarial Pyrimethamine Resistance via Allele-Specific PCR Coupled with Gold Nanoparticle-Based Lateral Flow Biosensor
Tingting Jiang, Yan Huang, Weijia Cheng, Yifei Sun, Wei Wei, Kai Wu, Chen‐Yang Shen, Xiaolong Fu, Haifeng Dong, Jian Li
Abstract
malaria patients. Compared with the results from nested PCR followed by Sanger DNA sequencing, the sensitivity was 97.96% (96/98) for N51I, C59R, and S108N. For specificity, the values were 100% (98/98), 95.92% (94/98), and 100% (98/98) for N51I, C59R, and S108N, respectively. The limit of detection is approximately 200 fg/μl for plasmid DNA as the template and 100 parasites/μl for blood filter paper. The established platform not only offers a powerful tool for molecular surveillance of ADR but also is easily extended to interrelated SNP profiles for infectious diseases and genetic diseases.
Topics & Concepts
Single-nucleotide polymorphismPyrimethamineAllelePolymorphism (computer science)BiologyGeneticsPolymerase chain reactionDrug resistanceBiosensorGenotypePlasmodium falciparumMalariaGeneBiochemistryImmunologyMalaria Research and ControlMosquito-borne diseases and controlvaccines and immunoinformatics approaches