Genome Editing of Veterinary Relevant Mycoplasmas Using a CRISPR-Cas Base Editor System
Thomas Ipoutcha, Fabien Rideau, Géraldine Gourgues, Yonathan Arfi, Carole Lartigue, Alain Blanchard, Pascal Sirand‐Pugnet
Abstract
. Several virulence factors were successfully targeted, and we were able to edit up to 54 target sites in a single step. The availability of this efficient and easy-to-use genetic tool will greatly facilitate functional studies of these economically important bacteria.
Topics & Concepts
CRISPRVirulenceBiologyGenomePathogenic bacteriaMicrobiologyGeneticsBacteriaGeneMicrobial infections and disease researchCRISPR and Genetic EngineeringPlant Virus Research Studies