MicroRNA‑100 functions as a tumor suppressor in cervical cancer via downregulating the SATB1 expression and regulating AKT/mTOR signaling pathway and epithelial‑to‑mesenchymal transition
Cuiping Huang, Xiaobo Qin, Na Zhao, Huijing Jin, Shuangjun Zhang, Haiyan Yang
Abstract
Cervical cancer (CC) is a common malignant tumor among women worldwide, remaining the fourth most frequent cause of cancer death in women. Currently, microRNA (miRNA) is a prevalent topic in tumor‑related research. The present study focused on the mechanisms of miR‑100 in CC progression. qRT‑PCR analysis revealed that the miR‑100 expression was notably decreased in CC tissues. In addition, miR‑100 downregulation was confirmed to be significantly related to the malignant clinicopathologic features of CC patients. Furthermore, miR‑100 overexpression was also verified to significantly repress CC cell proliferation, migration and invasion abilities through modulating the AKT/mTOR signaling pathway and epithelial‑to‑mesenchymal transition. Bioinformatics analysis and luciferase reporter assay identified that special AT‑rich sequence‑binding protein 1 was a functional target for miR‑100 in CC cells. Moreover, miR‑100 overexpression was found to markedly repress the CC tumor growth in vivo. In conclusion, the above results revealed that miR‑100 functioned as a cancer suppressor in CC progression and may provide insights into the novel therapeutic target for CC treatment.