Effect of surfactants on the production and biofunction of Tremella fuciformis polysaccharide through submerged fermentation
Caiyuan Yu, Fang Yan, Weiwei Huang, Peng Lei, Xiaoqi Xu, Dafeng Sun, Lingtian Wu, Hong Xu, Sha Li
Abstract
Tremella fuciformis polysaccharide (TFPS) have been widely used in the fields of food and medicine due to their multiple functions. submerged cultivation of mushroom is viewed as a promising alternative for efficient production of TFPS. Here, span 80 promoting the transfer of substrates and TFPS production were studied. The TFPS yield with addition of 0.3 % (v/v) span 80 in the culture reached 10.56 ± 0.53 g/L, which was 22.51 % higher than that of the control group. In a 7.5 L fermenter with glucose fed-batch strategy, the TFPS yield reached 25.94 ± 0.53 g/L. Span 80 improved the permeability of cell membrane and facilitated the secretion of TFPS. Furthermore, the glucuronic acid content of TFPS from Span 80 containing medium increased to 15.44 ± 0.15 %, and exhibited stronger antioxidant activity against DPPH• and •OH radicals. This study provided further evidence for elucidating the promoting effect of span 80 in submerged fermentation and guidance for the production of TFPS from T. fuciformis spore.