Annexin A1–dependent tethering promotes extracellular vesicle aggregation revealed with single–extracellular vesicle analysis
Maximillian A. Rogers, Fabrizio Buffolo, Florian Schlotter, Samantha K. Atkins, Lang Ho Lee, Arda Halu, Mark C. Blaser, Elena Tsolaki, Hideyuki Higashi, Kristin Luther, George G. Daaboul, Carlijn V. C. Bouten, Simon C. Body, Sasha A. Singh, Sérgio Bertazzo, Peter Libby, Masanori Aikawa, Elena Aïkawa
Abstract
knockdown attenuated EV aggregation and microcalcification formation in human cardiovascular cells and acellular three-dimensional collagen hydrogels. Our findings explain why microcalcifications are more prone to form in vulnerable regions of plaque, regulating critical cardiovascular pathology, and likely extend to other EV-associated diseases, including autoimmune and neurodegenerative diseases and cancer.
Topics & Concepts
TetheringExtracellular vesicleVesicleExtracellularCell biologyExtracellular vesiclesChemistryExtracellular matrixAnnexin A2AnnexinBiophysicsMicrovesiclesBiologyBiochemistryMembraneCellGenemicroRNAExtracellular vesicles in diseaseConnexins and lens biologyCell Adhesion Molecules Research