Molecular profiling of stem cell-derived retinal pigment epithelial cell differentiation established for clinical translation
Sandra Petrus-Reurer, Alex R. Lederer, Laura Baqué-Vidal, Iyadh Douagi, Belinda Pannagel, Irina Khven, Monica Aronsson, Hammurabi Bartuma, Magdalena Wagner, Andreas Wrona, Paschalis Efstathopoulos, Elham Jaberi, Hanni Willenbrock, Yutaka Shimizu, J. Carlos Villaescusa, Helder André, Erik Sundström, Aparna Bhaduri, Arnold R. Kriegstein, Anders Kvanta, Gioele La Manno, Fredrik Lanner
Abstract
retinal progenitor population and showed the ability of the protocol to suppress non-RPE fates. We demonstrated that the method produces a pure RPE pool capable of maturing further after subretinal transplantation in a large-eyed animal model. Our evaluation of hESC-RPE differentiation supports the development of safe and efficient pluripotent stem cell-based therapies for AMD.