A hydrophilic magnetic MOF for the consecutive enrichment of exosomes and exosomal phosphopeptides
Ning Zhang, Nianrong Sun, Chunhui Deng
Abstract
Hydrophilic Fe3O4@PDA@UIO-66-NH2 is employed to consecutively enrich urinary exosomes and exosomal phosphopeptides through the strong bond between Zr and phosphate groups. The recovery of exosomes reached 95% and 707 phosphopeptides were identified from 255 phosphoproteins via LC-MS/MS. This work will make a great contribution to further functional analysis of exosomes.
Topics & Concepts
MicrovesiclesBiocompatible materialChemistryNanoparticleExosomeNanotechnologyChemical engineeringMaterials scienceBiomedical engineeringBiochemistryMedicinemicroRNAEngineeringGeneExtracellular vesicles in diseaseAdvanced Nanomaterials in CatalysisCharacterization and Applications of Magnetic Nanoparticles