Three-channel smartphone-based aptamer sensor for multiplexed detecting antibiotics in water through resonance light scattering
Fengjing Cao, Hong-Huan Cheng, Shixiang Ma, Fu Jiao, Daming Dong
Abstract
A more sensitive, low-cost, and miniaturized three-channel smartphone-based aptamer sensor using resonance light scattering (RLS) as a readout method was designed to in-situ detect multiplexed antibiotics in water. In this system, three unlabeled aptamers were used to respectively recognize the targets and a smartphone with a three-channel design was the detector to obtain the corresponding RLS signal of aggregation AuNPs caused by depressed aptamer. The strategy simultaneously tested tobramycin (TOB), kanamycin (KANA), and alternariol (AOH) to verify the sensor performance using three-channel design. This sensor obtained good limit of detection of 24.23 ng/mL for TOB, and 58.03 ng/mL, and 0.18 μg/mL for KANA and AOH, linear ranges of 50–300 ng/mL, 200–1000 ng/mL, and 0.75–3.5 μg/mL, respectively. The recoveries for TOB ranged from 91.6%–105.4% and the coefficient of variation for the relative standard deviation was less than 10%. Compared with commonly used bio-sensors, this sensor has advantages of high-throughput assay and simple operation and realized three antibiotics detection using RLS as a signal output based on a miniaturized smartphone with a three-channel design.