Substrate stiffness reduces particle uptake by epithelial cells and macrophages in a size-dependent manner through mechanoregulation
Aaron Lee, Mauro Sousa de Almeida, Daela Milinkovic, Dedy Septiadi, Patricia Taladriz‐Blanco, Céline Loussert-Fonta, Sandor Balog, Amélie Bazzoni, Barbara Rothen‐Rutishauser, Alke Petri‐Fink
Abstract
characterization of A549 cell spreading and focal adhesion maturation. Consequently, decreasing substrate rigidity and particle-based topography led to a reduction of focal adhesion size. Moreover, expression levels of Yes-associated protein were found to correlate with the degree of particle endocytosis. A thorough appreciation of the mechanical cues may lead to improved solutions to optimize nanomedicine approaches for treatment of cancer and other diseases with abnormal mechanosignalling.
Topics & Concepts
Substrate (aquarium)StiffnessParticle sizeParticle (ecology)BiophysicsMaterials scienceChemistryNanotechnologyChemical engineeringComposite materialBiologyEcologyEngineeringPhysical chemistryBlood properties and coagulationCell Adhesion Molecules ResearchCellular Mechanics and Interactions