HexSDF Is Required for Synthesis of a Novel Glycolipid That Mediates Daptomycin and Bacitracin Resistance in C. difficile
Anthony G. Pannullo, Ziqiang Guan, Howard Goldfine, Craig D. Ellermeier
Abstract
Clostridioides difficile is a major cause of hospital-acquired diarrhea and represents an urgent concern due to the prevalence of antibiotic resistance and the rate of recurrent infections. Little is understood about C. difficile membrane lipids, but a unique glycolipid, HNHDRG, has been previously identified in C. difficile and, currently, has not been identified in other organisms. Here, we show that HexSDF and HexRK are required for synthesis of HNHDRG and that production of HNHDRG impacts resistance to daptomycin and bacitracin.
Topics & Concepts
DaptomycinGlycolipidBacitracinMicrobiologyDiarrheaClostridioidesClostridium difficileC difficileAntibioticsDrug resistanceMedicineVancomycinBacteriaBiologyImmunologyInternal medicineStaphylococcus aureusGeneticsClostridium difficile and Clostridium perfringens researchHelicobacter pylori-related gastroenterology studiesMicroscopic Colitis