Cell Cycle Arrest and Apoptotic Effect of 7-(4-(N-substituted carbamoylmethyl) piperazin-1-yl) Ciprofloxacin-derivative on HCT 116 and A549 Cancer Cells
Rania Alaaeldin, Maiiada Nazmy, Mohamed Abdel‐Aziz, Gamal El‐Din A. Abuo‐Rahma, Moustafa Fathy
Abstract
BACKGROUND/AIM: Ciprofloxacin has been used as an antibiotic in the clinic for decades. Recently, ciprofloxacin and its derivatives have shown promising anti-proliferative and cytotoxic activities against several malignant cells. The aim of this study was to investigate the effect of a new derivative of ciprofloxacin on colorectal cancer (HCT116) and non-small lung carcinoma (A549) cells. MATERIALS AND METHODS: Cell viability was detected by the MTT assay. Flow cytometry was used to examine the cell cycle and apoptosis. Expression of bax, bcl2, p53 and p21 was investigated by qRT-PCR and western blotting. RESULTS: /M phase and apoptosis. p53 and Bax proteins were overexpressed, while p21 and bcl2 gene expression was decreased after treatment with the ciprofloxacin derivative. CONCLUSION: This new ciprofloxacin derivative can be potentially used for the treatment of colorectal cancer and non-small lung carcinoma.