Extraction and purification of anthocyanins from <i>Peristrophe bivalvis</i> (L.) <i>Merr.</i> leaf (Acanthaceae) using aqueous two-phase systems
Phuong Hong Le, Duy N. Dao, Tin Q. Huynh, Tra T. T. Tran, Viet Nguyen
Abstract
(Acanthaceae) is a Vietnamese herb that is rich in anthocyanin, a natural colorant. However, the common extraction methods for this pigment often accompany with the presence of contaminated compounds. This study, therefore, focused on applying the aqueous two-phase system (ATPS), a green method for extracting and purifying anthocyanin in the leaf extract. The obtained results revealed that an ATPS composing from ethanol and ammonium sulfate with the concentrations of 32.5% and 25%, respectively, can recover 80% anthocyanin in leaf while eliminating 80% contaminated nitrogen compounds. Besides, the shelf-life of pigment in the purified extract was also elongated significantly in comparison with that in the crude extract.