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Diagnostic Performance of Xpert MTB/RIF Ultra Compared with Predecessor Test, Xpert MTB/RIF, in a Low TB Incidence Setting: a Retrospective Service Evaluation

Mary Mansfield, Anne Marie McLaughlin, Emma Roycroft, Lorraine Montgomery, Joseph Keane, Margaret Fitzgibbon, Thomas R. Rogers

2022Microbiology Spectrum14 citationsDOIOpen Access PDF

Abstract

The study demonstrates the improved sensitivity of the Ultra compared with the Xpert, particularly in smear negative TB disease, for both pulmonary and extrapulmonary samples in a low TB incidence setting. Cycle threshold (Ct) value for both assays was found to positively correlate with time to TB culture positivity, suggesting that Ct and semiquantitative results could be used as indicators of sample MTBC bacillary burden, and thus, perhaps, of transmission potential. This may have implications for the designation of patient isolation precautions.

Topics & Concepts

MedicineExtrapulmonary tuberculosisIncidence (geometry)TuberculosisPulmonary tuberculosisMycobacterium tuberculosis complexMycobacterium tuberculosisInternal medicineDiagnostic testRetrospective cohort studyVeterinary medicinePathologyOpticsPhysicsTuberculosis Research and EpidemiologyDiagnosis and treatment of tuberculosisPneumonia and Respiratory Infections
Diagnostic Performance of Xpert MTB/RIF Ultra Compared with Predecessor Test, Xpert MTB/RIF, in a Low TB Incidence Setting: a Retrospective Service Evaluation | Litcius