Risk Factors for Enteric Pathogen Exposure among Children in Black Belt Region of Alabama, USA
Drew Capone, Toheedat Bakare, Troy Barker, Amy Hutson Chatham, Ryan Clark, Lauren Copperthwaite, Abeoseh Flemister, Riley Geason, Emery Hoos, Elizabeth Kim, Alka Manoj, S. Pomper, Christina Samodal, Simrill Smith, Claudette Poole, Joe Brown
Abstract
We collected stool from school-age children from 352 households living in the Black Belt region of Alabama, USA, where sanitation infrastructure is lacking. We used quantitative reverse transcription PCR to measure key pathogens in stool that may be associated with water and sanitation, as an indicator of exposure. We detected genes associated with >1 targets in 26% of specimens, most frequently Clostridioides difficile (6.6%), atypical enteropathogenic Escherichia coli (6.1%), and enteroaggregative E. coli (3.9%). We used generalized estimating equations to assess reported risk factors for detecting >1 pathogen in stool. We found no association between lack of sanitation and pathogen detection (adjusted risk ratio 0.95 [95% CI 0.55–1.7]) compared with specimens from children served by sewerage. However, we did observe an increased risk for pathogen detection among children living in homes with well water (adjusted risk ratio 1.7 [95% CI 1.1–2.5]) over those reporting water utility service.