Cas3 Protein—A Review of a Multi-Tasking Machine
He Liu, Michael St. John James, Marin Radovčić, Ivana Ivančić-Baće, Edward L. Bolt
Abstract
Cas3 has essential functions in CRISPR immunity but its other activities and roles, in vitro and in cells, are less widely known. We offer a concise review of the latest understanding and questions arising from studies of Cas3 mechanism during CRISPR immunity, and highlight recent attempts at using Cas3 for genetic editing. We then spotlight involvement of Cas3 in other aspects of cell biology, for which understanding is lacking-these focus on CRISPR systems as regulators of cellular processes in addition to defense against mobile genetic elements.
Topics & Concepts
CRISPRComputational biologyGenome editingBiologyGeneticsGeneCRISPR and Genetic EngineeringGenetics, Aging, and Longevity in Model OrganismsInnovation and Socioeconomic Development