Serial optical coherence microscopy for label-free volumetric histopathology
Eunjung Min, Sungbea Ban, Junwon Lee, Andrey Vavilin, Song‐Yee Baek, Sunwoo Jung, Yujin Ahn, Kibeom Park, Sung-Won Shin, SoHyun Han, HyungJoon Cho, Whaseon Lee‐Kwon, Jeehyun Kim, C. Justin Lee, Woonggyu Jung
Abstract
The observation of histopathology using optical microscope is an essential procedure for examination of tissue biopsies or surgically excised specimens in biological and clinical laboratories. However, slide-based microscopic pathology is not suitable for visualizing the large-scale tissue and native 3D organ structure due to its sampling limitation and shallow imaging depth. Here, we demonstrate serial optical coherence microscopy (SOCM) technique that offers label-free, high-throughput, and large-volume imaging of ex vivo mouse organs. A 3D histopathology of whole mouse brain and kidney including blood vessel structure is reconstructed by deep tissue optical imaging in serial sectioning techniques. Our results demonstrate that SOCM has unique advantages as it can visualize both native 3D structures and quantitative regional volume without introduction of any contrast agents.