Fabrication of a dual-response intelligent antibacterial nanofiber and its application in beef preservation
Lin Lin, Jingjing Wu, Changzhu Li, Xiaochen Chen, Haiying Cui
Abstract
Cinnamaldehyde (CA) possesses high antibacterial activity, but it's unstable and volatile in food system. To overcome its drawbacks, a kind of dual-response controlled-release nanofiber was fabricated by electrospinning to embed [email protected] acid/carboxymethyl chitosan nanoparticles ([email protected]/CMCS-NPs) into silk fibroin nanofibers ([email protected]/CMCS-NPs SF nanofibers). When the concentration of [email protected]/CMCS-NPs was 5 mg/mL, [email protected]/CMCS-NPs SF nanofibers have better mechanical properties, the tensile strength was 3.21 MPa, and the elongation at break was 47.51%. The β-glucuronidase (β-G) and serine protease specifically secreted by Escherichia coli (E. coli) O157:H7 could deconstruct the shell of SF and HA via hydrolysis. It results in the dual-response controlled-release of CA from [email protected]/CMCS-NPs SF nanofibers. After 5 days of treatment with [email protected]/CMCS-NPs SF nanofibers at 4 °C and 25 °C, the amount of E. coli O157:H7 in beef was reduced by 99.98% and 99.89%, respectively. Besides, the [email protected]/CMCS-NPs SF nanofibers have no impact on the sensory quality of beef.