Targeted gene deletion in Brettanomyces bruxellensis with an expression-free CRISPR-Cas9 system
Cristián Varela, Caroline Bartel, Cristóbal A. Onetto, Anthony R. Borneman
Topics & Concepts
Genome editingCRISPRBiologyCas9Homologous recombinationGeneticsGeneSaccharomyces cerevisiaeGene targetingTransformation (genetics)GenomeYeastComputational biologyPloidyCRISPR and Genetic EngineeringFungal and yeast genetics researchGenetically Modified Organisms Research