Circularly permuted AsLOV2 as an optogenetic module for engineering photoswitchable peptides
Lequn Geng, Jiaqi Shen, Wenjing Wang
Abstract
We re-engineered a commonly-used light-sensing protein, AsLOV2, using a circular permutation strategy to allow photoswitchable control of the C-terminus of a peptide. We demonstrate that the circularly permuted AsLOV2 can be used on its own or together with the original AsLOV2 for enhanced caging. In summary, circularly permuted AsLOV2 could expand the engineering capabilities of optogenetic tools.
Topics & Concepts
PhotoswitchOptogeneticsProtein engineeringChemistryCombinatorial chemistryComputer scienceBiologyNeuroscienceBiochemistryPhotochemistryEnzymePhotochromic and Fluorescence ChemistryPhotoreceptor and optogenetics researchCRISPR and Genetic Engineering