High-yield vesicle-packaged recombinant protein production from E. coli
Tara A. Eastwood, Karen Baker, Bree R. Streather, Nyasha Allen, Lin Wang, Stanley W. Botchway, Ian R. Brown, Jennifer R. Hiscock, Christopher Lennon, Daniel P. Mulvihill
Abstract
We describe an innovative system that exports diverse recombinant proteins in membrane-bound vesicles from E. coli. These recombinant vesicles compartmentalize proteins within a micro-environment that enables production of otherwise challenging insoluble, toxic, or disulfide-bond containing proteins from bacteria. The release of vesicle-packaged proteins supports isolation from the culture and allows long-term storage of active protein. This technology results in high yields of vesicle-packaged, functional proteins for efficient downstream processing for a wide range of applications from discovery science to applied biotechnology and medicine.