A gold nanoparticle-based lateral flow immunosensor for ultrasensitive detection of tetrodotoxin
Yue Li, Xinxin Xu, Liqiang Liu, Hua Kuang, Liguang Xu, Chuanlai Xu
Abstract
in crucian and clam matrices without time-consuming pretreatment. Spiked samples were analyzed using the immunosensor and liquid chromatography-tandem mass spectrometry (LC-MS/MS). The immunosensor gave average intra-assay recoveries of 84.0-103.9%, inter-assay recoveries of 76.1-105% for the crucian matrix and intra-assay recoveries of 78.5-93.0%, inter-assay recoveries of 74.8-85.7% for the clam matrix. The results obtained using the immunosensor and LC-MS/MS were in good agreement. The immunosensor test could be completed within 10 min, offering a convenient option or complementarity to the traditional methods of TTX detection.
Topics & Concepts
TetrodotoxinNeurotoxinColloidal goldNanotechnologyNanoparticleChemistryBiosensorBiophysicsMaterials scienceBiologyBiochemistryMarine Toxins and Detection MethodsAdvanced biosensing and bioanalysis techniquesMolecular Sensors and Ion Detection