In Vivo Metabolic Imaging of [1‐ <sup>13</sup> C]Pyruvate‐d <sub>3</sub> Hyperpolarized By Reversible Exchange With Parahydrogen**
Henri de Maissin, Philipp Groß, Obaid Mohiuddin, Moritz Weigt, Luca Nagel, Marvin Herzog, Zirun Wang, Robert Willing, Wilfried Reichardt, M. Pichotka, Lisa Heß, Thomas Reinheckel, Henning J. Jessen, Robert Zeiser, Michael Bock, Dominik von Elverfeldt, Maxim Zaitsev, Sergey Korchak, Stefan Glöggler, Jan‐Bernd Hövener, Eduard Y. Chekmenev, Franz Schilling, Stephan Knecht, Andreas B. Schmidt
Abstract
Abstract Metabolic magnetic resonance imaging (MRI) using hyperpolarized (HP) pyruvate is becoming a non‐invasive technique for diagnosing, staging, and monitoring response to treatment in cancer and other diseases. The clinically established method for producing HP pyruvate, dissolution dynamic nuclear polarization, however, is rather complex and slow. Signal Amplification By Reversible Exchange (SABRE) is an ultra‐fast and low‐cost method based on fast chemical exchange. Here, for the first time, we demonstrate not only in vivo utility, but also metabolic MRI with SABRE. We present a novel routine to produce aqueous HP [1‐ 13 C]pyruvate‐d 3 for injection in 6 minutes. The injected solution was sterile, non‐toxic, pH neutral and contained ≈30 mM [1‐ 13 C]pyruvate‐d 3 polarized to ≈11 % (residual 250 mM methanol and 20 μM catalyst). It was obtained by rapid solvent evaporation and metal filtering, which we detail in this manuscript. This achievement makes HP pyruvate MRI available to a wide biomedical community for fast metabolic imaging of living organisms.