Human viral nucleic acids concentrations in wastewater solids from Central and Coastal California USA
Alexandria B. Boehm, Marlene K. Wolfe, Krista R. Wigginton, Amanda Bidwell, Bradley J. White, Bridgette Hughes, Dorothea Duong, Vikram Chan-Herur, Heather N. Bischel, Colleen C. Naughton
Abstract
We measured concentrations of SARS-CoV-2, influenza A and B virus, respiratory syncytial virus (RSV), mpox virus, human metapneumovirus, norovirus GII, and pepper mild mottle virus nucleic acids in wastewater solids at twelve wastewater treatment plants in Central California, USA. Measurements were made daily for up to two years, depending on the wastewater treatment plant. Measurements were made using digital droplet (reverse-transcription-) polymerase chain reaction (RT-PCR) following best practices for making environmental molecular biology measurements. These data can be used to better understand disease occurrence in communities contributing to the wastewater.