A highly efficient soybean transformation system using GRF3‐GIF1 chimeric protein
Zhao Ying, Peng Cheng, Ying Liu, C. Liu, Zhenbang Hu, Dawei Xin, Xiaoxia Wu, Mingliang Yang, Qingshan Chen
Abstract
Expression of GRF3-GIF1 chimera significantly enhanced regeneration and transformation efficiency in soybean, increasing the number of transformable cultivars. Moreover, GmGRF3-GIF1 can be combined with CRISPR/Cas9 for highly effective gene editing.
Topics & Concepts
Transformation (genetics)Fusion proteinComputational biologyComputer scienceBiologyGeneticsGeneRecombinant DNAPlant Molecular Biology ResearchPlant nutrient uptake and metabolismPlant tissue culture and regeneration