Retrospective Review of Clinical Utility of Shotgun Metagenomic Sequencing Testing of Cerebrospinal Fluid from a U.S. Tertiary Care Medical Center
Kyle G. Rodino, Michel Toledano, Andrew P. Norgan, Bobbi S. Pritt, Matthew J. Binnicker, Joseph D. Yao, Allen J. Aksamit, Robin Patel
Abstract
Shotgun metagenomic sequencing can detect nucleic acids from bacteria, fungi, viruses, and/or parasites in clinical specimens; however, little data exist to guide its optimal application to clinical practice. We retrospectively reviewed results of shotgun metagenomic sequencing testing requested on cerebrospinal fluid samples submitted to an outside reference laboratory from December 2017 through December 2019. Of the 53 samples from Mayo Clinic patients, 47 were requested by neurologists, with infectious diseases consultation in 23 cases.
Topics & Concepts
MetagenomicsShotgun sequencingClinical microbiologyShotgunTertiary careMedicineDNA sequencingCerebrospinal fluidBiologyPathologyMicrobiologyInternal medicineGeneticsGeneBacterial Infections and VaccinesInfectious Diseases and TuberculosisMycobacterium research and diagnosis