Food Safety Concerns in Pakistan: Monitoring of Antimicrobial-Resistant Bacteria and Residue Contamination in Commercially Available Fish and Poultry Meat Samples
Muhammad Usman Qamar, Kaneez Fizza, Muhammad Ismail Chughtai, Muhammad Shafique, Bibigul Seytkhanova, Ayaz Yktiyarov, Aatika Aatika, Zikria Saleem, Sana Mustafa, Zainab Tufail, Mahnoor Chaudhry, Tawaf Ali Shah, Ahmad Mohammad Salamatullah, Mohammed Bourhia
Abstract
Food safety is a critical issue in the 21st century due to antibiotic-resistant bacteria causing harmful foodborne diseases. This comprehensive study meticulously examined the presence of bacterial isolates, quinolone residue, and antimicrobial resistance genes in samples of broiler and fish meat. Forty samples were collected from various locations in the Faisalabad metropolis. The samples underwent culture on various types of agar, and the Vitek 2 compact system was used to confirm the isolates along with determining the minimum inhibitory concentration of antibiotics. Additionally, polymerase chain reaction was used to identify antimicrobial-resistant genes, and an enzyme-linked immunosorbent assay (ELISA) kit was employed to detect quinolone residue in each sample. Out of 40 fish samples, 66 polymicrobials were identified with Escherichia coli being the most prevalent (26.6%), followed by Klebsiella pneumoniae (16.6%) and Acinetobacter baumannii (15%). Furthermore, 70 polymicrobial were identified in the broiler samples, with 41.6% E. coli and 15% K. pneumoniae . In fish isolates, 14 (21%) qnr gene, 18 (27.2%) bla CTX-M , and 11 (16.6%) bla NDM-1 , and in broiler samples, 19 (27.1%) qnr gene, 19 (27.1%) bla TEM and 22 (31.4%) bla CTX-M , and 5 (7.1%) bla NDM-1 were found. Eighteen (45%) broiler and 7 (17.5%) fish samples with more than 100 µg per kg (ppb) for quinolone residues. The study concluded that the increased prevalence of multidrug-resistant bacteria and quinolone residue poses a significant threat to consumer health.