Construction of a Mitochondria‐Endoplasmic Reticulum Dual‐Targeted Red‐Emitting Fluorescent Probe for Imaging Peroxynitrite in Living Cells and Zebrafish
Lingchao He, Heng Liu, Jinsheng Wu, Ziyi Cheng, Fabiao Yu
Abstract
Abstract Peroxynitrite (ONOO − ) is one of the important reactive oxygen species, which plays a vital role in the physiological process of intracellular redox balance. Revealing the biological functions of ONOO − will contribute to further understanding of the oxidative process of organisms. In this work, we designed and synthesized a novel red‐emitting fluorescent probe MCSA for the detection of ONOO − , which could rapidly respond to ONOO − within 250 s and exhibited high sensitivity to ONOO − with a low detection limit of 78 nM. Co‐localization experiments demonstrated MCSA had the ability to localize into the mitochondria and endoplasmic reticulum. What's more, MCSA enabled monitoring ONOO − level changes during tunicamycin‐induced endoplasmic reticulum stress. We have also successfully achieved the visual detection of exogenous and endogenous ONOO − in living cells and zebrafish. This work presented a chemical tool for imaging ONOO − in vitro and in vivo.