Oxidative regulation of chloroplast enzymes by thioredoxin and thioredoxin-like proteins in <i>Arabidopsis thaliana</i>
Yuichi Yokochi, Yuka Fukushi, Ken‐ichi Wakabayashi, Keisuke Yoshida, Toru Hisabori
Abstract
Significance Plants modulate photosynthesis activity in response to the surrounding environment. It is well known that the redox-responsive protein thioredoxin (Trx) activates photosynthesis-related enzymes in the light. However, the factors involved in deactivating them are not well understood. Recent in vitro experiments suggest that several Trx and Trx-like proteins serve as oxidation factors for Trx-targeted proteins; thus, we examined their functions in vivo. Consequently, we found that f -type Trx and two types of Trx-like proteins, Trx-like 2 and atypical Cys His-rich Trx, were involved in oxidative deactivation of photosynthesis-related enzymes (e.g., fructose-1,6-bisphosphatase, Rubisco activase, and the ATP synthase γ-subunit). Thus, this study reveals the functions of oxidation factors in vivo and elucidates the regulation system for photosynthesis in the dark.