<i>In situ</i> assessment of mitochondrial respiratory activity and lipid metabolism of mouse oocytes using resonance Raman spectroscopy
Mika Ishigaki, Shinsuke Kashiwagi, Satoru Wakabayashi, Yumi Hoshino
Abstract
, the active respiration and lipid metabolism, means to catch the most fundamental biochemical reactions of life activities. Our results demonstrate the potential application of resonance Raman spectroscopy as a new, noninvasive, and universal cell evaluation technology, for not only oocytes but also more general cells such as somatic cells and iPS cells.
Topics & Concepts
Raman spectroscopyRespirationChemistryLipid dropletResonance (particle physics)In situCytochrome cMitochondrionMetabolismCytoplasmBiophysicsBiochemistryBiologyAnatomyPhysicsParticle physicsOpticsOrganic chemistrySpectroscopy Techniques in Biomedical and Chemical ResearchSpectroscopy and Chemometric AnalysesBee Products Chemical Analysis