High affinity Na ⁺ transport by wheat HKT1;5 is blocked by K ⁺

Abstract

Abstract The wheat sodium transporters TmHKT1;5‐A and TaHKT1;5‐D are encoded by genes underlying the major shoot Na + exclusion loci Nax2 and Kna1 from Triticum monococcum (Tm) and Triticum aestivum (Ta), respectively. In contrast to HKT2 transporters that have been shown to exhibit high affinity K + ‐dependent Na + transport, HKT1 proteins have, with one exception, only been shown to catalyze low affinity Na + transport and no K + transport. Here, using heterologous expression in Xenopus laevis oocytes we uncover a novel property of HKT1 proteins, that both TmHKT1;5‐A and TaHKT1;5‐D encode dual (high and low) affinity Na + ‐transporters with the high‐affinity component being abolished when external K + is in excess of external Na + . Three‐dimensional structural modeling suggested that, compared to Na + , K + is bound more tightly in the selectivity filter region by means of additional van der Waals forces, which is likely to explain the K + block at the molecular level. The low‐affinity component for Na + transport of TmHKT1;5‐A had a lower K m than that of TaHKT1;5‐D and was less sensitive to external K + . We propose that these properties contribute towards the improvements in shoot Na + ‐exclusion and crop plant salt tolerance following the introgression of TmHKT1;5‐A into diverse wheat backgrounds.

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