Size-selective DNA nanocage-based activatable CRISPR-Cas12a for sensitive and accurate detection of mature microRNA
Xiaoyi Fu, Yao Yin, Meng Zhang, Fangqi Peng, Yuyan Shi, Yan Liu, Yin Tan, Zilong Zhao, Xia Yin, Jie Song, Guoliang Ke, Xiaobing Zhang
Abstract
The sensitive and accurate detection of mature miRNA without the signal interference by pre-miRNAs is highly important. Herein, a size-selective DNA nanocage-based activatable CRISPR/Cas12a system was developed to achieve this goal.
Topics & Concepts
CRISPRNanocagesDNAmicroRNAChemistryComputational biologyMolecular biologyNanotechnologyBiologyGeneMaterials scienceBiochemistryCatalysisAdvanced biosensing and bioanalysis techniquesCRISPR and Genetic EngineeringRNA Interference and Gene Delivery