Chemical composition, enantiomeric analysis and anticholinesterase activity of <i>Lepechinia betonicifolia</i> essential oil from Ecuador
James Calva, Luis Cartuche, Salomé González, José Vinicio Montesinos, Vladimir Morocho
Abstract
CONTEXT: (Lam) Epling (Lamiaceae), a native shrub from Ecuador, was assessed. Chemical profiling and enantiomeric distribution were also recorded for the first time. OBJECTIVE: . MATERIALS AND METHODS: fresh aerial parts was obtained by hydrodistillation in a Clevenger-type apparatus. Physical properties were determined according to standard norms. The chemical composition was determined by GC-MS and GC-FID. Enantioselective GC-MS analysis was carried out by using a capillary chiral column. Anticholinesterase effect was assessed by Ellman's method with acetylthiocoline as substrate and Ellman's reagent (DTNB) to detect its hydrolysis at 405 nm for 60 min. Donepezil was used as a reference drug. EO was dissolved in methanol to reach 10 mg/mL concentration and two more 10× dilutions were included. RESULTS: value of 74.97 ± 1.17 μg/mL. DISCUSSION AND CONCLUSIONS: encourage us to propose this EO as a candidate for the preparation of functional foods or as adjuvant therapy for Alzheimer's disease.