A novel quinoline-based fluorescent probe for real-time monitoring of Cys in glioma
Shang-Ming-Zhu Zeng, Zhang Qing, Qin Li, Liangchao Yuan, Muhammad Abbas, Zhenxiang He, Hai‐Liang Zhu, Zhong‐Chang Wang
Abstract
experiments showed that ZS-C1 could be distinguished from Hcy and GSH significantly, and the fluorescence quantum yield was reduced by 30 times. Further, biological imaging and 3D tumor sphere penetration assay showed that the ZS-C1 could monitor both exogenous and endogenous Cys in the living U87MG cells, and the fluorescence of probe ZS-C1 diffusely distributed inside the U87MG three-dimensional solid cell spheroid (up to 60 μM deep into the solid tumors). This work provided a potential tool for further investigations of Cys in biological samples and critical information for early diagnosis of glioma and guidance for clinical surgery.