Visualizing Cholesterol in the Brain by On-Tissue Derivatization and Quantitative Mass Spectrometry Imaging
Roberto Angelini, Eylan Yutuc, Mark F. Wyatt, Jillian Newton, Fowzi Adam Yusuf, Lauren Griffiths, Benjamin Cooze, Dana El Assad, Gilles Frache, Wei Rao, Luke B. Allen, Željka Korade, Thu T. A. Nguyen, Rathnayake A. C. Rathnayake, Stephanie M. Cologna, Owain W. Howell, Malcolm R. Clench, Yuqin Wang, William J. Griffiths
Abstract
null mouse, confirming hypomyelination at the molecular level. Our study demonstrates the potential of MSI to the study of sterols in neuroscience.
Topics & Concepts
Mass spectrometry imagingChemistryCholesterolDerivatizationMass spectrometrySterolCorpus callosumPonsBrain tissueBiochemistryInternal medicineChromatographyNeurosciencePsychologyMedicineAdvanced Proteomics Techniques and ApplicationsGlycosylation and Glycoproteins ResearchMetabolomics and Mass Spectrometry Studies