Palladium–peptide oxidative addition complexes for bioconjugation
Anthony J. Rojas, Justin M. Wolfe, Heemal H. Dhanjee, Ivan Buslov, Nicholas L. Truex, Richard Y. Liu, Walter Massefski, Bradley L. Pentelute, Stephen L. Buchwald
Abstract
high-performance liquid chromatography. These complexes react with thiols in aqueous buffer, offering an efficient method for bioconjugation. Using this strategy, peptides can be functionalized with small molecules to prepare modified aryl thioether side-chains at low micromolar concentrations. Additionally, peptide-peptide and peptide-protein ligations are demonstrated under dilute aqueous conditions.
Topics & Concepts
BioconjugationPalladiumPeptideOxidative phosphorylationChemistryCombinatorial chemistryBiochemistryCatalysisChemical Synthesis and AnalysisClick Chemistry and ApplicationsPeptidase Inhibition and Analysis