Ex vivo capillary-parenchymal arteriole approach to study brain pericyte physiology
Danielle A Jeffrey, Jackson T. Fontaine, Fabrice Dabertrand
Abstract
Significance: two-photon microscopy. However, neither method adequately captures mural cell behavior without interfering neuronal tissue. Thus, there is a need to isolate vessels with their respective mural cells to study functional and pathological changes. Aim: method that recapitulates vessel dynamics in the brain. Approach: signals. Lastly, isolated microvasculature was cultured in DMEM media (up to 72 h), mounted, and pressurized using our CaPA preparation. Results: remained unchanged by culture conditions adding another application of longer treatment to our approach. Conclusion: CaPA methodology facilitates observation of arteriolar SMC and pericyte dynamic changes in real-time without environmental factors. This method will help to better understand how mural cells differ based on microvasculature location.