A novel DNA biosensor for the ultrasensitive detection of DNA methyltransferase activity based on a high-density “hot spot” SERS substrate and rolling circle amplification strategy
Shengjie Ge, Menglin Ran, Yu Mao, Yue Sun, Xinyu Zhou, Li Li, Xiaowei Cao
Abstract
, respectively. Finally, in order to verify the feasibility of its clinical application, the serum samples of healthy subjects and breast cancer, prostate cancer, gastric cancer and cervical cancer patients were analyzed, and the reliability of the results was also confirmed by western blot (WB) experiments. Taking advantage of these merits, the proposed biosensor can be a very promising alternative tool for the detection of M.SssI activity, which is of vital importance in the early detection and prevention of tumors.
Topics & Concepts
Rolling circle replicationBiosensorSubstrate (aquarium)DNAMethyltransferaseHot spot (computer programming)DNA methyltransferaseCpG siteChemistryMaterials scienceNanotechnologyMolecular biologyBiochemistryBiologyDNA methylationComputer scienceGeneDNA polymeraseMethylationGene expressionEcologyOperating systemAdvanced biosensing and bioanalysis techniquesNanocluster Synthesis and ApplicationsBiosensors and Analytical Detection