Chromatin expansion microscopy reveals nanoscale organization of transcription and chromatin
Mark E. Pownall, Liyun Miao, Charles E. Vejnar, Ons M’Saad, Alice Sherrard, Megan A. Frederick, Maria D.J. Benitez, Curtis W. Boswell, Kenneth S. Zaret, Joerg Bewersdorf, Antonio J. Giráldez
Abstract
Nanoscale chromatin organization regulates gene expression. Although chromatin is notably reprogrammed during zygotic genome activation (ZGA), the organization of chromatin regulatory factors during this universal process remains unclear. In this work, we developed chromatin expansion microscopy (ChromExM) to visualize chromatin, transcription, and transcription factors in vivo. ChromExM of embryos during ZGA revealed how the pioneer factor Nanog interacts with nucleosomes and RNA polymerase II (Pol II), providing direct visualization of transcriptional elongation as string-like nanostructures. Blocking elongation led to more Pol II particles clustered around Nanog, with Pol II stalled at promoters and Nanog-bound enhancers. This led to a new model termed "kiss and kick", in which enhancer-promoter contacts are transient and released by transcriptional elongation. Our results demonstrate that ChromExM is broadly applicable to study nanoscale nuclear organization.