NIR-II Responsive Nanohybrids Incorporating Thermosensitive Hydrogel as Sprayable Dressing for Multidrug-Resistant-Bacteria Infected Wound Management
Weilun Pan, Bodeng Wu, Chengtao Nie, Tingting Luo, Zhenli Song, Jie Lv, Yong Tan, Chunchen Liu, Mingzhen Zhong, Tong Liao, Z. Wang, Guanghui Yi, Limin Zhang, Xiaoliu Liu, Bo Li, Jin-Xiang Chen, Lei Zheng
Abstract
Developing an effective dressing against bacterial infection and synchronously addressing wound complications, such as bleeding, long-term inflammation, and reinfection, are highly desirable in clinical practice. In this work, a second near-infrared (NIR-II) responsive nanohybrid consisting of i mipenem encapsulated l iposome with g old-shell and lipopolysaccharide (LPS)-targeting a ptamer, namely ILGA, is constructed for bacteria elimination. Benefiting from the delicate structure, ILGA exhibits strong affinity and a reliable photothermal/antibiotic therapeutic effect toward multidrug-resistant Pseudomonas aeruginosa ( MDR-PA ). Furthermore, by incorporating ILGA with a thermosensitive hydrogel poly(lactic- co -glycolic acid)–polyethylene glycol–poly(lactic- co -glycolic acid) (PLGA-PEG-PLGA), a sprayable dressing ILGA@Gel was prepared, which enables a quick on-demand gelation (10 s) for wound hemostasis and offers excellent photothermal/antibiotic efficacy to sterilize the infected wound. Additionally, ILGA@Gel provides satisfactory wound-healing environments by reeducating wound-associated macrophages for inflammation alleviation and forming a gel layer to block exogenous bacterial reinfection. This biomimetic hydrogel reveals excellent bacteria eradication and wound recovery effectiveness, demonstrating its promising potential for managing complicated infected wounds.