Construction of tumor organoids and their application to cancer research and therapy
Jiajing Lv, Xuan Du, Miaomiao Wang, Jiacan Su, Yan Wei, Can Xu
Abstract
3D tumor model to mimic the pathophysiological characteristics of parental tumors. Various techniques have been developed to construct tumor organoids, such as matrix-based methods, hanging drop, spinner or rotating flask, nonadhesive surface, organ-on-a-chip, 3D bioprinting, and genetic engineering. This review elaborated on cell components and fabrication methods for establishing tumor organoid models. Furthermore, we discussed the application of tumor organoids to cancer modeling, basic cancer research, and anticancer therapy. Finally, we discussed current limitations and future directions in employing tumor organoids for more extensive applications.
Topics & Concepts
OrganoidCancer therapyTumor microenvironmentCancerTumor heterogeneityTumor cellsCancer researchCancer cellComputational biologyMedicineBiologyNeuroscienceInternal medicine3D Printing in Biomedical ResearchCancer Cells and MetastasisCancer Research and Treatments