Dehalogenimonas etheniformans sp. nov., a formate-oxidizing, organohalide-respiring bacterium isolated from grape pomace
Yiru Cui, Xiuying Li, Jun Yan, Yan Lv, Huijuan Jin, Jingjing Wang, Gao Chen, Fadime Kara Murdoch, Yi Yang, Frank E. Löffler
Abstract
A strictly anaerobic, organohalide-respiring bacterium, designated strain GP T , was characterized using a polyphasic approach. GP T is Gram-stain-negative, non-spore-forming and non-motile. Cells are irregular cocci ranging between 0.6 and 0.9 µm in diameter. GP T couples growth with the reductive dechlorination of 1,2-dichloroethane, vinyl chloride and all polychlorinated ethenes, except tetrachloroethene, yielding ethene and inorganic chloride as dechlorination end products. H 2 and formate serve as electron donors for organohalide respiration in the presence of acetate as carbon source. Major cellular fatty acids include C 16 : 0 , C 18 : 1 ω9 c , C 16 : 1 , C 14 : 0 and C 18 : 0 . On the basis of 16S rRNA gene phylogeny, GP T is most closely related to Dehalogenimonas formicexedens NSZ-14 T and Dehalogenimonas alkenigignens IP3-3 T with 99.8 and 97.4 % sequence identities, respectively. Genome-wide pairwise comparisons based on average nucleotide identity, average amino acid identity and digital DNA–DNA hybridization do not support the inclusion of GP T in previously described species of the genus Dehalogenimonas with validly published names. On the basis of phylogenetic, physiological and phenotypic traits, GP T represents a novel species within the genus Dehalogenimonas , for which the name Dehalogenimonas etheniformans sp. nov. is proposed. The type strain is GP T (= JCM 39172 T = CGMCC 1.17861 T ).