Engineered dsRNA–protein nanoparticles for effective systemic gene silencing in plants
Huayu Sun, Ankarao Kalluri, Dan Tang, Jingwen Ding, Longmei Zhai, Xianbin Gu, Yanjun Li, Huseyin Yer, Xiaohan Yang, Gerald A. Tuskan, Zhanao Deng, Frederick G. Gmitter, Hui Duan, Challa V. Kumar, Yi Li, Yi Li, Yi Li
Abstract
Abstract Long-distance transport or systemic silencing effects of exogenous biologically active RNA molecules in higher plants have not been reported. Here, we report that cationized bovine serum albumin (cBSA) avidly binds double-stranded beta-glucuronidase RNA (dsGUS RNA) to form nucleic acid–protein nanocomplexes. In our experiments with tobacco and poplar plants, we have successfully demonstrated systemic gene silencing effects of cBSA/dsGUS RNA nanocomplexes when we locally applied the nanocomplexes from the basal ends of leaf petioles or shoots. We have further demonstrated that the cBSA/dsGUS RNA nanocomplexes are highly effective in silencing both the conditionally inducible DR5-GUS gene and the constitutively active 35S-GUS gene in leaf, shoot, and shoot meristem tissues. This cBSA/dsRNA delivery technology may provide a convenient, fast, and inexpensive tool for characterizing gene functions in plants and potentially for in planta gene editing.