Porous Silicon-Based Aptasensors: Toward Cancer Protein Biomarker Detection
Sofia Arshavsky‐Graham, Simon J. Ward, Naama Massad‐Ivanir, Thomas Scheper, Sharon M. Weiss, Ester Segal
Abstract
(0.2 μM), which is attributed to mass transfer limitations. To improve the latter, we applied different strategies to increase the diffusion flux to and within the nanostructure, such as the application of isotachophoresis for the preconcentration of AGR2 on the aptasensor, mixing, or integration with microchannels. By combining these approaches with a new signal processing technique that employs Morlet wavelet filtering and phase analysis, we achieve a limit of detection of 15 nM without compromising the biosensor's selectivity and specificity.
Topics & Concepts
AptamerDetection limitBiosensorPancreatic cancerChemistryMaterials sciencePorous siliconBiomarkerLinear rangeNanotechnologyChromatographySiliconCancerMolecular biologyOptoelectronicsBiologyBiochemistryGeneticsSilicon Nanostructures and PhotoluminescenceAdvanced biosensing and bioanalysis techniquesNanowire Synthesis and Applications